|
|
 |
 |
 |
|
|
|
Entry Options |
|
|
|
|
|
|
|
|
|
| Link to related information: |
 |
|
|
| Save entry: |
 |
|
|
|
| View: |
 |
|
|
|
|
 |
|
 |
|
|
|
|
CABRI:DSMZ_MUTZ |
|
Accession_number |
ACC 305
| |
Cell_line_name |
293
| |
Brief_description |
confirmed as human with IEF of AST, LDH established from a human primary
embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category
1 according to the German Central Commission for Biological Safety (ZKBS); cell line
also known as HEK-293 (human embryonic kidney-293)
| |
Description |
human embryonal kidney established from a human primary embryonal kidney
transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the
German Central Commission for Biological Safety (ZKBS); cell line also known as
HEK-293 (human embryonic kidney-293) confirmed as human with IEF of AST, LDH
| |
Depositor |
Dr. W. Dirks, DSMZ, Braunschweig, Germany
| |
Morphology |
adherent fibroblastoid cells growing as monolayer
| |
Culture_conditions |
85% Dulbecco's MEM + 15% FBS split confluent culture 1:5 to 1:6 every 2-3 days
(without using trypsin/EDTA, cells can be detached by tapping the flask); seed out at
about 2-3 x 106 cells/25 cm 2 at 37C with 5-10% CO2 cell harvest of about 40 x
10 6 cells/175 cm 2
| |
Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
HTLV-I/II -, SMRV -
| |
Properties |
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 (+),
desmin -, endothel -, EpCAM -, GFAP -, neurofilament +, vimentin +
| |
Release_conditions |
-
| |
Hazard |
1
| |
Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule
| |
Doubling_time |
about 24-30 hours
| |
Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
| |
Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
| |
Cytogenetics |
human near-triploid karyotype with 6% polyploidy - 69(64-69)XX?X, +1, +6, -10, -13,
-16, -18, -20, +21, +22, +mar, add(?X)(p22), inv(1)(p35q43), del(1)(p35), i(5p),
del(6)(q11), add(8)(q24), add(13)(p13), add(17)(qter), der(?)t(5;?)(q13;?)
| |
Bibliographic_reference |
Graham et al., J Gen Virol, 36: 9 (1977) [PMID: 886304
] |
|